Sterilisasi pada suhu 121oc selama 10 menit dengan tutup tabung dikendurkan. Distribute about 10 ml of the medium into middle size of test tube and sterilize by autoclaving at 121 c for 15 minutes. Samples are tested with cell suspensions of vibrio choleraeatcc 9458, diluted to the equivalent of a 0. Alkaline peptone water is used for detection of vibrio species. Alkaline saline peptone water aspw was first formulated by shread, donovan and lee to be used as a nonselective enrichment broth for the cultivation of aeromonas species. The purpose of this study was to ascertain the suitability of apw supplemented.
Alkaline peptone waterbased enrichment method for mcr3 from acute diarrheic outpatient gut samples. Alkaline peptone water intended use himedia laboratories. Its formulation conforms to iso 218721 and iso 218722. Alkaline peptone water safety data sheet date of issue 15062010. Safety data sheet sds alkaline peptone water certificates of analysis. A special medium for vibrio organisms is alkaline peptone water apw, which is both a transport and an enrichment medium. If you are viewing this page as a nonregistered user, the prices displayed is list price. In certain instances for example, when the patient is in very early stages of illness and is passing liquid. Kencangkan tutup tabung selama penyimpanan dan sesudah inokulasi. Peptone water is formulated as per shread, donovan and lee 9. However, culture method should remain for cholera outbreak.
Principle peptone supplies amino acids, nitrogen, carbon and other nutrients which support the growth of microorganism. Peptone water is a suitable enrichment broth for this purpose. Microxpress material safety data sheet according to 200158ec doc. This study compares recoveries of vibrio parahaemolyticus and vibrio vulnificus with saltpolymyxin. Dispense the medium in 10 ml amounts in screwcap bottles. Fresh stool samples and rectal swabs were incubated in 1% apw for 6 h at room temperature before testing with rdt following the manufacturers instruction.
Any of various water soluble protein derivatives formed by partial hydrolysis or digestion of proteins by an acid or enzyme, used in culture media in. Tubes are incubated with loose caps at 35 37c for one day in an aerobic atmosphere. Alkaline peptone water culture media online microbiology notes. Incubation at an elevated temperature 42c can further select for v. The alkaline peptone water was adjusted to a ph of 8. Alkaline peptone water dehydrated thermo fisher scientific. Alkaline saline peptone water aspw is in accordance with isots 218721. Pdf alkaline peptone waterbased enrichment method for mcr3. Prior to release, all lots of alkaline peptone water are tested to verify specific product characteristics. Pharmacopeia national formulary uspnf for use in pharmaceutical and cosmetic testing and by the american public health association and environmental protection agency for food, dairy and water industries. Alkaline saline peptone water iso is a liquid medium used for the enrichment of vibrio species from food and water samples. Early studies on animal models reported that alkaline water supplementation may exert positive effects on body weight improvement and development in offspring 1, 2. Alkaline saline peptone water iso was first described by shread, donovan, and lee 1 to be used as a nonselective enrichment broth for the cultivation of aeromonas species. The relatively high ph of the medium approximately 8.
Alkaline peptone water apw is recommended as an enrichment broth for isolating vibrio cholerae from clinical samples and non clinical. A number of other broth media have been described for enrichment of v. Can i use pancreatic digest of casein as peptone in. Recommended for enrichment of vibrio species from food and water samples in accordance with iso. Alkaline peptone water, 8ml, 16x125mm tube, order by the package of 20, by hardy diagnostics minimum order. Alkaline saline peptone water aspw was first formulated by shread, donovan and lee 3 to be used as a nonselective enrichment broth for the cultivation of aeromonas species. Accidental release measures spill sweep up into suitable containers for recovery or disposal. Alkaline peptone water for microbiology find msds or sds, a coa, data sheets and more information. Cruickshank reported that the raised ph of the medium could be used to effectively cultivate vibrio species 2. Alkaline peptone water is generally used as an enrichment medium in the isolation of vibrio spp.
Peptone water definition of peptone water by the free. Enrichment in apw enhances the isolation of choleraev. May 31, 2016 alkaline water, often referred to as alkaline ionized water akw, is commercially available and is mainly proposed for electrolyte supplementation during intensive perspiration. Clinical samples like swabs and faeces in food and. Msds a015 bis section 1 product and company information page product name. This medium is recommended by apha 8 for enrichment of vibrio species from seafood, infectious materials and other. Alkaline peptone water is a broth medium for the enrichment of. Cm1117 a broth medium for the enrichment of vibrio species from food and water samples. Alkaline peptone water is a broth medium for the enrichment of vibrio species from food, water and clinical samples. Dec 21, 2010 peptone 10 g nacl 10 g aquadest 1 liter larutkan semua bahan dalam aquadest dan panaskan perlahanlahan. These samples should be dilufold increments to 10ted in 103 to reduce the numbers of competing microorganisms.
Alkaline saline peptone water for microbiology aspw. Lab224 alkaline saline peptone water iso alkaline peptone water, alkaline saline water description alkaline saline peptone water iso is a medium for the enrichment of vibrio spp. Alkaline peptone water apw is recommended as an enrichment broth, and thiosulfate citrate bile salts sucrose tcbs agar is the selective agar medium of choice for isolating v. Frontiers alkaline peptone waterbased enrichment method. Us2590517a method for the preparation of peptone and amino.
Dissolve the peptone and sodium chloride in the water. Water samples for food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines 7. O1 when few organisms are present, as in specimens from convalescent patients and asymptomatic carriers. Alkaline peptone water iso for microbiology sigmaaldrich. Alkaline peptone water was first formulated by shread, donovan and lee1 to be used as a nonselective enrichment broth for the cultivation of aeromonas spp. Alkaline peptone water is a preenrichment medium specially standardized for vibrio. We conducted the study between march and june 2015. First developed by shread, donovan and lee as an enrichment broth for the growth of aeromonas spp. Alkaline peptone water is a suitable enrichment broth for this purpose 46. I am wondering if it is okay to use pancreatic digest of casein instead of peptic digest of animal tissue in preparing alkaline peptone water. This products resides on a fisher scientific gsa or va contract. This medium may also be used for the enrichment of vibrio spp. Alkaline saline peptone water for microbiology aspw sigma.
Aspw, alkaline peptone water with 2% sodium chloride. Aliquots 1 g of each stool sample were individually inoculated into 5 ml of alkaline peptone water binhe, hangzhou, china and 5 ml of luriabertani lb broth for enrichment overnight at 35c. Peptone water is recommended 3,6,7 for studying the ability of an organism to ferment a specific carbohydrate which aid in differentiation of genera and species. An enrichment medium for the cultivation of vibrio spp. Alkaline peptone water apw and thiosulfate citrate bile salts sucrose tcbs agar are the most widely used media for isolation of v. Alkaline saline peptone water aspw was first formulated by shread, donovan and lee to be used as a nonselective enrichment broth for the. Alkaline peptone water enrichment with a dipstick test to. Enrichment medium for isolation of vibrio spp from food and water samples, according to iso 21872 all. Thiosulfate citrate bile salts sucrose tcbs agar 5.
List of culture media used in microbiology with their uses. Stool specimens enriched in alkaline peptone water or luriabertani lb broth were screened for mcr1, mcr2, and mcr3 using polymerase. Us2590517a method for the preparation of peptone and. Nov 21, 2017 our findings showed that the modified rdt test after enrichment with 1% alkaline peptone water had high level of accuracy in detection of v. Samples are tested with cell suspensions of vibrio choleraeatcc 9458, diluted to the equivalent of a. Cm1028 personal precautions wear appropriate protective clothing. Thermo scientific remel alkaline peptone water ph 8. Alkaline peptone water was first formulated by shread, donovan and lee 1 to be used as a nonselective enrichment broth for the cultivation of aeromonas spp.
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